Hogeschool Leiden

Workshop metagenomics

For the Dutch version of this course text click here.

Metagenomics studies the presence of micro organisms in a sample and uncovers the interactions between those micro organisms. A great advantage of metagenomics is that it can - in principle - access 100% of the genetic material of a sample, whereas traditional cultivation and genomics methods access only a fraction. By revealing the secrets of microbial communities, metagenomics has been responsible for advances in biomedicine, biotechnology, agriculture and ecology.

Topics

  • workflow of sample preparation, DNA isolation, selection of sequencing technology, read mapping, annotation and metagenome assembly;
  • 16S and metagenome analyses;
  • taxonomic classification;
  • quantification of microbial communities and species richness (so-called alpha diversity);
  • review of functional properties of the microorganisms in the samples by adding annotations and via metabolic pathway reconstruction;
  • pipelines for data analysis and presentation of the data.

Learning objectives

During this course you will learn:

  • the basic principles and concepts needed to understand the basics of metagenomics research;
  • the basic workflow in a lab conducting of metagenomic studies and assays;
  • how to take samples and isolate DNA for metagenomics analysis and what the common pitfalls are;
  • how to analyze 16S and metagenome data with freely accessible online tools;
  • how to interpret the results and present the data.

Practical information

Programme
Draft programme metagenomics ( pdf, 117 KB )

For whom
laboratory professionals who are involved in metagenomics research but who have limited experience with the relevant techniques or who are outsourcing their metagenomics analysis

Course level
hbo

Language
English

Course dates
27th and 28th of November 2017

Course hours
09.30 - 16.00h

Extent
14 hours theory

Certificate
Proof of participation

Registration
before 23rd of October 2017

Course fee
€ 1.020,- (by registration before 18th of September 2017 € 920,-)

Registration

You can registrate here.

All our courses can be arranged as "customized programs".
For questions you can contact us by phone 071-5188743/753 or via cbd@hsleiden.nl.

Back to courses Genomics or Moleculaire Biologie